Application Note: RapidVap Vertex Evaporator
LinkProtocol:
US EPA Method 550.1 requires an evaporation or concentration step when extracting polyaromatic hydrocarbons from liquid. The data presented are compounds from the matrix spike.
Provisions:
RapidVap Vertex Evaporator (Cat#7320020/SN-111150275 A) with 40 mL block.
40 mL I-Chem vials with PTFE lined septa.
Chromatographic grade Methylene Chloride and Acetonitrile.
Agilent 1100 HPLC with Ultraviolet and Fluorescence Detection
Polyaromatic Hydrocarbon standards purchase from Supelco Analytical.
Procedure:
40mL vials were filled with 10.0mL of methylene chloride spiked with 5ng/mL – 100ng/mL depending on the concentration of the analytical standard and concentrated down to 1mL.
The block preheat and temperature was 40°C with 10 p.s.i. of nitrogen. The 0.5mL was brought to 3mL with acetonitrile and then concentrated to 0.5mL.
Three preparations were injected in duplicate and compared to a 1ug/mL standard of polyaromatic hydrocarbons for percent recovery determinations.
Table 1 (below) shows the recoveries of the matrix spike. Method 550.1 was used as a reference.
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